Lonza AMAXA Lonza Nucleofector II 細(xì)胞核轉(zhuǎn)染儀
單孔細(xì)胞核轉(zhuǎn)染儀
Ÿ 優(yōu)化的電穿孔技術(shù)
Ÿ 獨(dú)特的細(xì)胞特異性電轉(zhuǎn)染程序
Ÿ 程序的不斷更新升級(jí)
Lonza(原Amaxa)公司的Nucleofection®技術(shù)在傳統(tǒng)的電穿孔技術(shù)基礎(chǔ)上取得了突破性進(jìn)展，通過(guò)調(diào)整優(yōu)化電轉(zhuǎn)參數(shù)（內(nèi)存轉(zhuǎn)染程序），結(jié)合細(xì)胞特異性的轉(zhuǎn)染試劑直接把外源基因?qū)朐?xì)胞和傳代細(xì)胞的細(xì)胞漿和細(xì)胞核中。在保證高效轉(zhuǎn)染的同時(shí)，也提高了細(xì)胞的存活率；
世界上**直接把外源基因?qū)爰?xì)胞核的**型技術(shù)！
世界上**能高效轉(zhuǎn)染原代細(xì)胞、懸浮細(xì)胞的非病毒轉(zhuǎn)染技術(shù)！
世界上**能在數(shù)小時(shí)內(nèi)完成從實(shí)驗(yàn)操作到結(jié)果分析全過(guò)程的轉(zhuǎn)染技術(shù)！
全球共享細(xì)胞優(yōu)化轉(zhuǎn)染程序數(shù)據(jù)庫(kù)；
高效轉(zhuǎn)染DNA、RNA、peptide等各種底物；
已發(fā)表超過(guò)5000篇文獻(xiàn)（www.lonza.com/nucleofection-citations ）。
全球公認(rèn)的*高效轉(zhuǎn)染技術(shù)，它綜合應(yīng)用傳統(tǒng)的電穿孔技術(shù)及細(xì)胞特異性的細(xì)胞核轉(zhuǎn)染液，通過(guò)調(diào)整優(yōu)化電轉(zhuǎn)參數(shù)（內(nèi)存程序），直接把外源基因?qū)朐?xì)胞和傳代細(xì)胞核中。針對(duì)**細(xì)胞、神經(jīng)細(xì)胞、干細(xì)胞等幾乎所有難轉(zhuǎn)染的細(xì)胞系或原代細(xì)胞或懸浮細(xì)胞。
Transfection is an essential tool for numerous applications, including studies of gene expression. However, transfection of cells with common methods such as calcium phosphate, DEAE-dextran, liposome-mediated transfer, electroporation or viral vectors is limited because of low transfection efficiency and the extended time required for analysis of gene expression. Amaxa Biosystems has a new electroporation-based transfection method that enables the DNA to directly enter the nucleus while cells are stabilized in an electric field by treatment with a specific solution. The method is called “Nucleofection” and the electoporator system is called Nucleofector II. The system has built-in optimized programs for most common cell types.
The transfection procedure is simple and straightforward with these basic steps: (1) Preparing cells to be transfected with the nucleofection solution appropriate for the cell type, (2) Adding the nucleic acid to the prepared cells and transferring the mixture to a cuvette (provided in the kit), (3) Placing the cuvette in the system, electroporating cells with the optimized program for the cell type, (4) Harvesting cells from the cuvette and dispersing on to media contained in a dish. The whole process can be done in less than 30 minutes.
The good part about the system is that the method is easy, simple (some optimization required with regard to the appropriate amount of DNA and the type of program used for each cell type), sensitive, requires minimal expertise and no prior plating of cells to be transfected is needed. This in contrast to the alternative methods which are limited by the need to plate cells at a particular density 24-48 hours prior to transfection. In addition, these methods are labor and time intensive. Amaxa has nucleofection kits with optimized protocols available for most common cell types. As nucleic acids are delivered straight into the nucleus, nucleofected cells can be ready for analysis after 2–6 hrs of transfection.